• Contact Information

  • Publication Information

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    (only papers published in the last 5 years will be allowed)
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    Accepted file types: pdf, docx, doc, Max. file size: 10 MB.
      (only papers published in English considered)
      (only full length articles with detailed materials and methods sections will be considered; no reviews, meta-analyses/systematic analyses, short communications, etc. will be considered))
    • Study Information

    • For each question below, add in the line number and page number in the manuscript where the given information can be found OR add in a detailed explanation for why this information is not included in the manuscript.

    • Study Design
      For each experiment, provide brief details of study design including:
      a. The groups being compared, including control groups. If no control group has been used, the rationale should be stated.
      b. The experimental unit (e.g., a single animal, litter, or cage of animals).

    • Sample Size
      a. Specify the exact number of experimental units allocated to each group, and the total number in each experiment. Also indicate the total number of animals used.
      b. Explain how the sample size was decided. Provide details of any a priori sample size calculation, if done.

    • Inclusion and exclusion criteria
      a. Describe any criteria established a priori for including and excluding animals (or experimental units) during the experiment, and data points during the analysis.
      b. For each experimental group, report any animals, experimental units or data points not included in the analysis and explain why.
      c. For each analysis, report the exact value of N in each experimental group.

    • Randomization: Describe the methods used:
      a. To allocate experimental units to control and treatment groups. If randomization was used, provide the method of randomization.
      b. To minimize potential confounding factors such as the order of treatments and measurements, or animal/cage location.

    • Outcome measures
      a. Clearly define all outcome measures assessed (e.g. cell death, molecular markers, or behavioral changes).
      b. For hypothesis-testing studies, specify the primary outcome measure, i.e. the outcome measure that was used to determine the sample size.

    • Statistical methods
      a. Provide details of the statistical methods used for each analysis.
      b. Specify the experimental unit that was used for each statistical test.
      c. Describe any methods used to assess whether the data met the assumptions of the statistical approach.

    • Experimental animals
      a. Provide details of the animals used, including species, strain and substrain, sex, age or developmental stage, and weight.
      b. Provide further relevant information on the provenance of animals, health/immune status, genetic modification status, genotype, and any previous procedures.

    • Experimental procedures
      For each experimental group, including controls, describe the procedures in enough detail to allow others to replicate them, including:
      a. What was done, how it was done and what was used.
      b. When and how often.
      c. Where (including detail of any acclimation periods).
      d. Why (provide rationale for procedures).

    • Results
      For each experiment conducted, including independent replications, report:
      a. Summary/descriptive statistics for each experimental group, with a measure of variability where applicable.
      b. If applicable, the effect size with a confidence interval.
    • This field is for validation purposes and should be left unchanged.